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Diffusion and Osmosis: How Substances Move In and Out of Cells — Answers

Answer sheet with model answers and success criteria for the Year 9 cells & classification worksheet.

Success TutoringWorksheetsAnswer sheetYear 9 · Science · Cells & Classification

Diffusion and Osmosis: How Substances Move In and Out of Cells — Answers

Part A: Diffusion

Particles in liquids and gases are always moving randomly. Diffusion is the net movement of particles from where they are more concentrated to where they are less concentrated, until they are spread evenly.

Cells rely on diffusion. Oxygen diffuses from the blood into cells, where it is used in respiration, and carbon dioxide diffuses out. Diffusion is faster when the temperature is higher, the concentration difference is larger and the distance is shorter.

1.Diffusion is the net movement of particles from an area of (answer space) concentration to an area of lower concentration.

Answer: higher

Particles spread out until they are evenly mixed.

2.A drop of food colouring is placed in a beaker of still water. Which change would make it spread out fastest?
  1. AUsing warmer water
  2. BUsing colder water
  3. CUsing a taller beaker
  4. DAdding the drop more slowly

Answer: Using warmer water

Particles move faster at higher temperatures, so diffusion is faster.

3.Name one substance that diffuses into your body cells and one that diffuses out of them.

Answer: In: oxygen (or glucose). Out: carbon dioxide (or other wastes such as urea).

4.Diffusion needs the cell to use energy.

Answer: False

Diffusion is passive: it happens because of the random movement of particles, so no energy from the cell is needed.

Part B: Osmosis

The cell membrane is partially permeable: it lets small molecules such as water through but stops larger ones. Osmosis is the movement of water across a partially permeable membrane from a dilute solution (more water) to a concentrated solution (less water).

Plant cells that take in water become firm, or turgid, because the cell wall resists. Plant cells that lose water become soft, or flaccid. Animal cells have no cell wall, so they can burst if too much water enters.

5.Osmosis is the movement of which substance across a partially permeable membrane?
  1. ASalt
  2. BSugar
  3. CWater
  4. DOxygen

Answer: Water

Osmosis is a special case of diffusion involving water only.

6.A red blood cell is placed in pure water. What happens?
  1. AWater moves in, and the cell swells and may burst
  2. BWater moves out, and the cell shrinks
  3. CNothing, because water cannot cross the membrane
  4. DSalt moves into the cell

Answer: Water moves in, and the cell swells and may burst

The cell contents are more concentrated than pure water, so water moves in. Animal cells have no cell wall, so the cell may burst.

7.A plant cell is placed in pure water. Water moves in, but the cell does not burst. Explain why.

Answer: The strong cell wall stops the cell from expanding too far. The cell becomes firm (turgid) instead of bursting.

8.Explain why a lettuce leaf left in very salty water becomes soft and limp.

Answer: The salty water is more concentrated than the cell contents, so water leaves the cells by osmosis. The vacuoles shrink and the cells lose their firmness, so the leaf goes limp.

Part C: Potato strip experiment

Students cut three potato strips, each with a mass of 5.0 g. They placed one strip in each solution for 30 minutes, dried them gently and measured their mass again.

Results: pure water 5.5 g; medium salt solution 4.6 g; strong salt solution 4.2 g.

Percentage change = (final mass − starting mass) ÷ starting mass × 100

9.Calculate the percentage change in mass for the strip in pure water.

Answer: +10%

  1. Change = 5.5 − 5.0 = +0.5 g
  2. 0.5 ÷ 5.0 × 100 = +10% (a gain)
10.Calculate the percentage change in mass for the strip in the medium salt solution.

Answer: −8%

  1. Change = 4.6 − 5.0 = −0.4 g
  2. −0.4 ÷ 5.0 × 100 = −8% (a loss)
11.Calculate the percentage change in mass for the strip in the strong salt solution.

Answer: −16%

  1. Change = 4.2 − 5.0 = −0.8 g
  2. −0.8 ÷ 5.0 × 100 = −16% (a loss)
12.Explain the pattern in the results using the word osmosis.

Answer: In pure water, water moved into the potato cells by osmosis, so the strip gained mass. In the salt solutions, water moved out of the cells by osmosis, and the stronger the solution, the more water was lost, so the greater the loss in mass.

13.Why did the students calculate percentage change rather than just comparing the final masses?

Answer: It allows a fair comparison even if the strips do not all start with exactly the same mass.

Part D: Why are cells so small?

14.A model cell is a cube with sides of 1 cm. Calculate its surface area, its volume and its surface area to volume ratio.

Answer: Surface area 6 cm², volume 1 cm³, ratio 6 : 1

  1. Surface area = 6 faces × (1 × 1) = 6 cm²
  2. Volume = 1 × 1 × 1 = 1 cm³
  3. Ratio = 6 : 1
15.Repeat for a cube with sides of 2 cm.

Answer: Surface area 24 cm², volume 8 cm³, ratio 3 : 1

  1. Surface area = 6 × (2 × 2) = 24 cm²
  2. Volume = 2 × 2 × 2 = 8 cm³
  3. Ratio = 24 : 8 = 3 : 1
16.Use your answers to explain why cells are very small, and why large organisms are made of many small cells rather than one giant cell.[4 marks]

Model answer: As a cube gets bigger, its volume increases faster than its surface area, so its surface area to volume ratio falls (6 : 1 for the 1 cm cube, but only 3 : 1 for the 2 cm cube). A cell takes in oxygen and nutrients and removes wastes through its surface by diffusion. A small cell has a large surface area compared with its volume, so substances can enter and leave quickly enough, and the distance to the centre is short. A giant cell would not have enough surface for its volume, and diffusion to its centre would be too slow, so large organisms are made of many small cells.

Success criteria:

  • States that the surface area to volume ratio decreases as size increases, using the calculated values
  • Links surface area to the exchange of substances by diffusion
  • Explains that diffusion over long distances is too slow
  • Concludes why organisms are made of many small cells
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