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WorksheetYear 8Level 2 · Standard

Free Year 8 Using a Microscope: Parts, Magnification and Slide Preparation Worksheet

Students learn the parts of a light microscope and what each one does, calculate total magnification, estimate the size of cells from the field of view, and order the steps for preparing a stained wet-mount slide of onion cells. They finish by explaining how to focus a microscope correctly and safely.

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Year
Year 8
Subject
Science
Topic
Cells & Classification
Difficulty
Level 2 · Standard
Estimated time
35 minutes
Curriculum
New Zealand Curriculum
Questions
14
Answers
Included, with working
Format
PDF (A4), print or complete online

Students will practise

  • naming the parts of a light microscope and their functions
  • calculating total magnification
  • estimating cell size from the field of view
  • preparing a wet-mount slide
  • focusing and handling a microscope safely

Curriculum: New Zealand Curriculum. We show specific outcome codes only where they have been verified against the official curriculum document.

What's next?

Completed: Using a Microscope: Parts, Magnification and Slide Preparation

  1. 1Plant and Animal Cells: Structures and Functions

How to use this worksheet

  1. Print the PDF or complete it on screen. Write the student's name and date at the top.
  2. Read the instructions for each part together before starting. Encourage working to be shown.
  3. Mark with the answer sheet. Use the model answers and success criteria as a guide rather than a single right answer.
  4. Record the score, celebrate what went well, then follow the "What's next" suggestion.

Common questions

Who is this worksheet for?

Year 8 students (typically ages 12–13) working on cells & classification. It is pitched at level 2 · standard.

Are the answers included?

Yes. The PDF includes a full answer sheet with model answers and success criteria where there is more than one good answer. You can also view the answers online.

How long does it take?

About 35 minutes for 14 questions. Short, regular sessions work best: two or three a week beats one long one.

Do I need to sign up to download?

No. Click Download Free PDF and it opens immediately. It is free for personal, classroom and homeschool use.

What should we do next?

Try Plant and Animal Cells: Structures and Functions.

Preview

Success TutoringWorksheetsYear 8 · Science · Cells & Classification

Using a Microscope: Parts, Magnification and Slide Preparation

Name

Date

Score /

Total magnification = eyepiece × objective. Always start on low power.

Part A: Parts of a microscope

Read the passage, then answer the questions.

A light microscope uses lenses to magnify small objects such as cells. Light shines up from a lamp or mirror through a thin specimen on a glass slide, then through the objective lens and the eyepiece lens to your eye.

Most school microscopes have three or four objective lenses on a turning nosepiece, often 4×, 10× and 40×. The coarse and fine focus knobs move the stage up and down to bring the image into focus.

1.Match each microscope part to its job.
  • eyepiece lens
  • objective lenses
  • stage
  • coarse focus knob
  • fine focus knob
  • diaphragm
  • controls how much light passes through the specimen
  • the lens you look through, usually magnifying 10 times
  • lenses of different strengths just above the slide
  • moves the stage a large distance to focus roughly
  • the flat platform that holds the slide
  • makes small adjustments to get a sharp image
2.Why should you always start observing with the lowest-power objective lens?
  1. AIt uses less electricity.
  2. BIt shows the largest area, so the specimen is easiest to find and focus on.
  3. CIt makes cells look bigger.
  4. DHigh-power lenses do not work first.
3.You should carry a microscope with one hand holding the arm and the other hand under the base. ☐ True   ☐ False
4.When using the high-power objective, you should use the coarse focus knob to make big adjustments. ☐ True   ☐ False

Part B: Magnification and cell size

Show your working.

Total magnification = eyepiece magnification × objective magnification.

The field of view is the circle of light you see. To estimate a cell's size, divide the width of the field of view by the number of cells that fit across it. Cells are usually measured in micrometres (µm): 1 mm = 1000 µm.

5.An eyepiece of 10× is used with an objective of 4×. What is the total magnification? (answer space)
6.What is the total magnification with a 10× eyepiece and a 40× objective? (answer space)
7.A microscope has a 15× eyepiece. Which objective gives a total magnification of 150×? (answer space)
8.The field of view is 2 mm across. About 10 cells fit side by side across it. Estimate the length of one cell in millimetres and in micrometres (1 mm = 1000 µm). (answer space)
9.When you switch from low power to high power, the field of view …
  1. Agets larger and shows more cells
  2. Bgets smaller and shows fewer cells, but each looks bigger
  3. Cstays exactly the same
  4. Dturns upside down for the first time

Part C: Preparing and viewing a slide

10.Put these steps in order to make a wet-mount slide of onion cells: A lower a coverslip at an angle; B peel a thin layer of skin from the inside of an onion layer; C add a drop of iodine stain; D lay the onion skin flat on the slide in a drop of water; E place the slide on the stage and view on low power. (answer space)
11.Why is the coverslip lowered slowly at an angle?
  1. Ato stop air bubbles being trapped
  2. Bto make the stain darker
  3. Cto make the onion skin thicker
  4. Dto warm the slide
12.A stain such as iodine is used so that parts of the cell, such as the (answer space), are easier to see.
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About this resource

Created by
Success Tutoring Science Team
Last reviewed
1 October 2026
How it was made
Written by our team

Free to print and share for personal, classroom and homeschool use. Please don't resell. Spotted an error? Let us know.