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Worksheet7th GradeLevel 2 · Standard

Free 7th Grade Biotic and Abiotic Factors in Ecosystems Worksheet

Students learn the difference between biotic (living) and abiotic (non-living) factors, how each affects where organisms live and how many there are, and which instruments are used to measure abiotic factors. They then use quadrat data to estimate a population size and explain how to make field sampling reliable.

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Grade
7th Grade
Subject
Science
Topic
Ecosystems & Food Chains
Difficulty
Level 2 · Standard
Estimated time
35 minutes
Curriculum
Texas grade levels (TEKS)
Questions
14
Answers
Included, with working
Format
PDF (A4), print or complete online

Students will practice

  • classifying factors as biotic or abiotic
  • explaining how environmental factors affect the distribution of organisms
  • choosing instruments to measure abiotic factors
  • estimating population size from quadrat data
  • explaining how to make field sampling reliable

Curriculum: Texas grade levels (TEKS). We show specific outcome codes only where they have been verified against the official curriculum document.

What's next?

Completed: Biotic and Abiotic Factors in Ecosystems

  1. 1Relationships in Ecosystems: Competition, Predation and Symbiosis
  2. 2Classification and Dichotomous Keys
  3. 3Using a Microscope: Parts, Magnification and Slide Preparation
  4. 4Plant and Animal Cells: Structures and Functions

How to use this worksheet

  1. Print the PDF or complete it on screen. Write the student's name and date at the top.
  2. Read the instructions for each part together before starting. Encourage working to be shown.
  3. Mark with the answer sheet. Use the model answers and success criteria as a guide rather than a single right answer.
  4. Record the score, celebrate what went well, then follow the "What's next" suggestion.

Common questions

Who is this worksheet for?

7th Grade students (typically ages 12–13) working on ecosystems & food chains. It is pitched at level 2 · standard.

Are the answers included?

Yes. The PDF includes a full answer sheet with model answers and success criteria where there is more than one good answer. You can also view the answers online.

How long does it take?

About 35 minutes for 14 questions. Short, regular sessions work best: two or three a week beats one long one.

Do I need to sign up to download?

No. Click Download Free PDF and it opens immediately. It is free for personal, classroom and homeschool use.

What should we do next?

Try Relationships in Ecosystems: Competition, Predation and Symbiosis.

Preview

Success TutoringWorksheets7th Grade · Science · Ecosystems & Food Chains

Biotic and Abiotic Factors in Ecosystems

Name

Date

Score /

Biotic = living. Abiotic = non-living.

Part A: Living or non-living?

Circle biotic or abiotic.

An ecosystem is made up of all the organisms in an area together with their non-living surroundings.

Biotic factors are the living parts of an ecosystem and their effects: food sources, predators, competitors, parasites and disease. Abiotic factors are the non-living parts: light, temperature, water, wind, soil type, soil pH and saltiness.

Both kinds of factor affect where an organism can live (its distribution) and how many there are (its population size).

1.The amount of sunlight reaching the forest floor is a(n) … factor.
  1. Abiotic
  2. Babiotic
2.Rabbits eating the grass in a field is a(n) … factor.
  1. Abiotic
  2. Babiotic
3.The saltiness of the water in a river mouth is a(n) … factor.
  1. Abiotic
  2. Babiotic
4.A disease spreading through a frog population is a(n) … factor.
  1. Abiotic
  2. Babiotic
5.Soil temperature is a(n) … factor.
  1. Abiotic
  2. Babiotic
6.All the living things and non-living surroundings in an area, interacting together, make up an (answer space).

Part B: Measuring the environment

7.Match each abiotic factor to the instrument used to measure it.
  • light intensity
  • air or soil temperature
  • soil pH
  • soil moisture
  • wind speed
  • pH test kit or pH meter
  • anemometer
  • moisture meter
  • thermometer or temperature probe
  • light meter
8.Moss grows thickly on the shady side of a wall but hardly at all on the sunny side. Suggest one abiotic factor that could explain this. (answer space)
9.A new predator arriving in an ecosystem is an example of a change in an abiotic factor. ☐ True   ☐ False

Part C: Sampling with quadrats

Show your working.

It is usually impossible to count every plant in a large area, so ecologists take samples. A quadrat is a square frame, often 1 m × 1 m, placed on the ground. The organisms inside it are counted.

Estimated population = mean number per quadrat × (total area ÷ area of one quadrat).

Field study: A class wanted to estimate how many clover plants grow in a grassy field with an area of 200 m². They placed a 1 m × 1 m quadrat at five random spots and counted the clover plants inside each one.

Quadrat 1: 4 · Quadrat 2: 6 · Quadrat 3: 3 · Quadrat 4: 7 · Quadrat 5: 5

10.What is the mean number of clover plants per quadrat? (answer space)
11.Estimate the total number of clover plants in the 200 m² field. (answer space)
12.Why were the quadrats placed randomly?
  1. Ato save time
  2. Bso the class did not choose only the areas with the most (or fewest) plants, which would bias the results
  3. Cbecause random places have more clover
  4. Dso they could skip counting
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Preview — the PDF contains the full resource and answer sheet.

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Ecosystems & Food Chains in other year levels

About this resource

Created by
Success Tutoring Science Team
Last reviewed
1 October 2026
How it was made
Written by our team

Free to print and share for personal, classroom and homeschool use. Please don't resell. Spotted an error? Let us know.