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Study Guide8th GradeLevel 2 · Standard

Free 8th Grade Plant and Animal Cells and the Light Microscope

A study guide to the structure of plant and animal cells: what each organelle does, which structures only plant cells have, and why not every plant cell is green. It also explains the parts of a light microscope, how to calculate total magnification and how to focus safely, with check-yourself questions and answers.

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Grade
8th Grade
Subject
Science
Topic
Cells & Classification
Difficulty
Level 2 · Standard
Estimated time
25 minutes
Curriculum
Texas grade levels (TEKS)
Answers
Not applicable
Format
PDF (A4) + print

Students will practice

  • naming the organelles of plant and animal cells and describing their functions
  • comparing plant and animal cells
  • naming the parts of a light microscope
  • calculating total magnification
  • using a microscope safely and focusing correctly

Curriculum: Texas grade levels (TEKS). We show specific outcome codes only where they have been verified against the official curriculum document.

What's next?

Completed: Plant and Animal Cells and the Light Microscope

  1. 1Specialized Cells, Tissues and Organs
  2. 2Diffusion and Osmosis: How Substances Move In and Out of Cells

How to use this study guide

  1. Read it together first, pausing at each worked example to try the step before reading the answer.
  2. Attempt the "Check yourself" questions at the end without looking back.
  3. Then practice with a worksheet from the pathway above and finish with the topic test.

Common questions

Who is this study guide for?

8th Grade students (typically ages 13–14) working on cells & classification. It is pitched at level 2 · standard.

Are the answers included?

This is a study guide, so there is no separate answer sheet; the 'Check yourself' questions include answers.

How long does it take?

About 25 minutes. Short, regular sessions work best: two or three a week beats one long one.

Do I need to sign up to download?

No. Click Download Free PDF and it opens immediately. It is free for personal, classroom and homeschool use.

What should we do next?

Try Specialized Cells, Tissues and Organs.

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Success TutoringWorksheets8th Grade · Science · Cells & Classification

Plant and Animal Cells and the Light Microscope

What is a cell?

The cell is the basic unit of life: every living thing is made of one or more cells. Some organisms, such as bacteria, are a single cell. Your body is made of trillions of cells of many different types. Most cells are far too small to see without a microscope.

Inside a cell are smaller structures called organelles, each with its own job. Plant and animal cells share many organelles, but plant cells have some extra ones.

Organelles and their functions

StructureFound inFunction
Cell membranePlant and animal cellsA thin, flexible layer that controls what enters and leaves the cell
CytoplasmPlant and animal cellsJelly-like fluid where many of the cell's chemical reactions take place
NucleusPlant and animal cellsContains the genetic material (DNA) and controls the cell's activities
MitochondriaPlant and animal cellsCarry out cellular respiration, releasing energy from glucose
RibosomesPlant and animal cellsMake proteins (too small to see with a school light microscope)
Cell wallPlant cells onlyA rigid layer made of cellulose, outside the membrane, that supports the cell and gives it shape
ChloroplastsPlant cells only (green parts)Contain the green pigment chlorophyll and carry out photosynthesis
Large permanent vacuolePlant cells onlyFilled with cell sap; keeps the cell firm. Animal cells have only small, temporary vacuoles, if any
Not every plant cell has chloroplasts. Root cells grow underground in the dark, so they cannot carry out photosynthesis and have no chloroplasts. They do still have a cell wall and a large vacuole.

Quick comparison. Both plant and animal cells have a cell membrane, cytoplasm, a nucleus, mitochondria and ribosomes. Only plant cells have a cell wall, chloroplasts and a large permanent vacuole. Animal cells are often rounded or irregular in shape, while plant cells are usually more regular, box-like shapes because of the cell wall.

The light microscope

PartWhat it does
Eyepiece lensThe lens you look through; it magnifies the image (often 10×)
Objective lensesTwo to four lenses of different strengths (for example 4×, 10× and 40×) that you rotate into place
Stage and clipsThe flat platform that holds the slide; the clips keep it still
Light source or mirrorShines light up through the specimen
Coarse focus knobMoves the stage or lens a long way, for first focusing at low power
Fine focus knobMakes small adjustments to sharpen the image

Calculating total magnification

  1. Total magnification = eyepiece magnification × objective magnification.
  2. Eyepiece 10× and objective 4×: 10 × 4 = 40×.
  3. Eyepiece 10× and objective 40×: 10 × 40 = 400×. The image looks 400 times larger than the real specimen.

Using a microscope well

  1. Carry the microscope with two hands: one on the arm and one under the base.
  2. Start with the lowest-power objective lens. It shows a wider area, so the specimen is easier to find.
  3. Looking from the side, raise the stage (or lower the lens) until the objective is close to the slide, without touching it.
  4. Look through the eyepiece and use the coarse focus knob to move the lens and slide apart until the image appears.
  5. Sharpen the image with the fine focus knob, then switch to a higher-power lens and use only the fine focus.
Stains such as iodine solution are used in the school laboratory to make parts of a cell, such as the nucleus, easier to see. Thin specimens, such as a single layer of onion skin, let light pass through.

Check yourself

  1. Name three structures found in plant cells but not in animal cells.
  2. Which organelle releases energy from glucose?
  3. What does the cell membrane do?
  4. An eyepiece is 10× and the objective lens is 40×. What is the total magnification?
  5. The total magnification is 100× and the eyepiece is 10×. What is the objective lens?
  6. Explain why root cells have no chloroplasts.
  7. A cell has a nucleus, a cell membrane and a cell wall, but no chloroplasts. Is it from a plant or an animal? Explain.
  8. Why should you always start with the lowest-power objective lens?

Answers: 1. Cell wall, chloroplasts and a large permanent vacuole. 2. The mitochondria (mitochondrion is the singular). 3. It controls what enters and leaves the cell. 4. 10 × 40 = 400×. 5. 100 ÷ 10 = 10×. 6. Roots are underground with no light, so they cannot carry out photosynthesis; chloroplasts would have no job there. 7. A plant, because animal cells never have a cell wall. It may be a root cell, which explains the missing chloroplasts. 8. It shows a wider area, so it is easier to find and focus on the specimen before zooming in.

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About this resource

Created by
Success Tutoring
Last reviewed
1 October 2026
How it was made
Written by our team

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