What is a cell?
The cell is the basic unit of life: every living thing is made of one or more cells. Some organisms, such as bacteria, are a single cell. Your body is made of trillions of cells of many different types. Most cells are far too small to see without a microscope.
Inside a cell are smaller structures called organelles, each with its own job. Plant and animal cells share many organelles, but plant cells have some extra ones.
Organelles and their functions
| Structure | Found in | Function |
|---|
| Cell membrane | Plant and animal cells | A thin, flexible layer that controls what enters and leaves the cell |
| Cytoplasm | Plant and animal cells | Jelly-like fluid where many of the cell's chemical reactions take place |
| Nucleus | Plant and animal cells | Contains the genetic material (DNA) and controls the cell's activities |
| Mitochondria | Plant and animal cells | Carry out cellular respiration, releasing energy from glucose |
| Ribosomes | Plant and animal cells | Make proteins (too small to see with a school light microscope) |
| Cell wall | Plant cells only | A rigid layer made of cellulose, outside the membrane, that supports the cell and gives it shape |
| Chloroplasts | Plant cells only (green parts) | Contain the green pigment chlorophyll and carry out photosynthesis |
| Large permanent vacuole | Plant cells only | Filled with cell sap; keeps the cell firm. Animal cells have only small, temporary vacuoles, if any |
Not every plant cell has chloroplasts. Root cells grow underground in the dark, so they cannot carry out photosynthesis and have no chloroplasts. They do still have a cell wall and a large vacuole.
Quick comparison. Both plant and animal cells have a cell membrane, cytoplasm, a nucleus, mitochondria and ribosomes. Only plant cells have a cell wall, chloroplasts and a large permanent vacuole. Animal cells are often rounded or irregular in shape, while plant cells are usually more regular, box-like shapes because of the cell wall.
The light microscope
| Part | What it does |
|---|
| Eyepiece lens | The lens you look through; it magnifies the image (often 10×) |
| Objective lenses | Two to four lenses of different strengths (for example 4×, 10× and 40×) that you rotate into place |
| Stage and clips | The flat platform that holds the slide; the clips keep it still |
| Light source or mirror | Shines light up through the specimen |
| Coarse focus knob | Moves the stage or lens a long way, for first focusing at low power |
| Fine focus knob | Makes small adjustments to sharpen the image |
Calculating total magnification
- Total magnification = eyepiece magnification × objective magnification.
- Eyepiece 10× and objective 4×: 10 × 4 = 40×.
- Eyepiece 10× and objective 40×: 10 × 40 = 400×. The image looks 400 times larger than the real specimen.
Using a microscope well
- Carry the microscope with two hands: one on the arm and one under the base.
- Start with the lowest-power objective lens. It shows a wider area, so the specimen is easier to find.
- Looking from the side, raise the stage (or lower the lens) until the objective is close to the slide, without touching it.
- Look through the eyepiece and use the coarse focus knob to move the lens and slide apart until the image appears.
- Sharpen the image with the fine focus knob, then switch to a higher-power lens and use only the fine focus.
Stains such as iodine solution are used in the school laboratory to make parts of a cell, such as the nucleus, easier to see. Thin specimens, such as a single layer of onion skin, let light pass through.
Check yourself
- Name three structures found in plant cells but not in animal cells.
- Which organelle releases energy from glucose?
- What does the cell membrane do?
- An eyepiece is 10× and the objective lens is 40×. What is the total magnification?
- The total magnification is 100× and the eyepiece is 10×. What is the objective lens?
- Explain why root cells have no chloroplasts.
- A cell has a nucleus, a cell membrane and a cell wall, but no chloroplasts. Is it from a plant or an animal? Explain.
- Why should you always start with the lowest-power objective lens?
Answers: 1. Cell wall, chloroplasts and a large permanent vacuole. 2. The mitochondria (mitochondrion is the singular). 3. It controls what enters and leaves the cell. 4. 10 × 40 = 400×. 5. 100 ÷ 10 = 10×. 6. Roots are underground with no light, so they cannot carry out photosynthesis; chloroplasts would have no job there. 7. A plant, because animal cells never have a cell wall. It may be a root cell, which explains the missing chloroplasts. 8. It shows a wider area, so it is easier to find and focus on the specimen before zooming in.